Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua

Journal of venomous animals and toxins

Ana Marisa Chudzinski-Tavassi¹  Ana Claudia Sato¹  Mauricio Barbugiani Goldfeder¹  o da Silva¹  Sonia Elisabete Alves Will¹  Bá¹  Kerly Fernanda Mesquita Pasqualoto²  Miryam Paola Alvarez-Flores²  Rosemary Viola Bosch²  rbara Athayde Vaz Galvã²  Sonia Aparecida de Andrade² 

[1] Laboratory of Biochemistry and Biophysics, Butantan Institute, São Paulo, Brazil

关键词: Hemolin;    rLosac;    Wound healing;    Collagen;   

DOI  :  10.1186/s40409-016-0093-4

学科分类：药理学

来源: BioMed Central

【 摘 要 】

Hemolin proteins are cell adhesion molecules from lepidopterans involved in a wide range of cell interactions concerning their adhesion properties. However, hemolin’s roles in cell proliferation and wound healing are not fully elucidated. It has been recently reported that rLosac, a recombinant hemolin from the caterpillar Lonomia obliqua, presents antiapoptotic activity and is capable of improving in vitro wound healing. Therefore, this study aimed to explore rLosac’s in vivo effects using a skin wound healing model in rats. Circular full-thickness wounds in the rat dorsum skin were treated either with rLosac, or with saline (control), allowing healing by keeping the wounds occluded and moist. During the wound healing, the following tissue regeneration parameters were evaluated: wound closure and collagen content. Furthermore, tissue sections were subjected to histological and immunohistochemical analyses. The rLosac treatment has demonstrated its capacity to improve wound healing, as reflected in findings of a larger number of activated fibroblasts, proliferation of epithelial cells, increase of collagen type 1, and decrease of inflammatory infiltrate. The findings have indicated the rLosac protein as a very promising molecule for the development of new wound-healing formulations.

【 授权许可】

CC BY