Stimulation of human keratinocyte growth by alginate oligosaccharides, a possible co‐factor for epidermal growth factor in cell culture

FEBS Letters

Hiura, Nozomi³  Shiraiwa, Masakazu²  Ishibashi, Akira¹  Tajima, Shingo¹  Hara, Kenji⁴  Kawada, Akira¹  Takahara, Hidenari²  Hiruma, Masataro¹ 

[1] Department of Dermatology, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359, Japan;Department of Bioresource Science, School of Agriculture, Ibaraki University, Ami, Ibaraki, Japan;Maruha Corporation Central Research Institute, Tsukuba, Ibaraki, Japan;Laboratory of Marine biochemistry, Faculty of Fisheries, Nagasaki University, Nagasaki, Japan

关键词: Alginate oligosaccharide;    Keratinocyte;    Proliferation;    Epidermal growth factor;    Bovine pituitary extract;    BPE;    bovine pituitary extract;    EGF;    epidermal growth factor;    bFGF;    basic fibroblast growth factor;    HS;    heparan sulfate;    Δ;    O-(4-deoxy-α-l-erythro-hex-4-enopyranosyluronic acid)-1→;    -GulA-;    →4)-O-(α-l-gulopyranosyluronic acid)-(1→;    -GulA;    →4)-O-β-l-gulopyranuronic acid;    -ManA-;    →4)-O-(β-d-mannopyranuronic acid)-(1→;    -ManA;    →4)-O-α-d-mannopyranuronic acid;    -ManA′;    →4)-O-β-d-mannuronic acid;   

DOI  :  10.1016/S0014-5793(97)00386-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oligosaccharides, involved in regulation of plant developmental and defensive processes, were tested to determine their ability to enhance proliferation of human keratinocytes. A mixture of alginate oligosaccharides remarkably stimulated keratinocyte growth and [³H]thymidine uptake in the presence of epidermal growth factor (EGF). The activity was comparable to bovine pituitary extract, a common complement in keratinocyte culture, and additive on BPE-induced stimulation. The most effective oligosaccharide in the mixture was identified and its chemical structure was determined. These findings demonstrate a novel activity of alginate oligosaccharide(s) in keratinocyte growth and suggest a possible co-factor for EGF-dependent stimulation in medium for keratinocytes.

【 授权许可】

Unknown