FEBS Letters | |
The G protein α subunit (GPα1) is associated with the ER and the plasma membrane in meristematic cells of Arabidopsis and cauliflower | |
Huang, Hai1  White, Elyssa1  Ma, Hong1  Weiss, Catherine A1  | |
[1] Cold Spring Harbor Laboratory, P.O. Box 100, Cold Spring Harbor, NY 11724, USA | |
关键词: Immunofluorescence; Cell fractionation; G protein; Plasma membrane; ER; Arabidopsis; Cauliflower; | |
DOI : 10.1016/S0014-5793(97)00378-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Towards the elucidation of the cellular function(s) of GPα1, we have characterized its subcellular localization using immunofluorescence and cell fractionation. GPα1 is not present in nuclei or chloroplasts. It is a membrane-bound protein, and analysis of isolated endoplasmic and plasma membranes indicates a good correlation between GPα1 in both the plasma membrane and the ER compartment. Interestingly, these results may suggest more different functions for GPα1: it might be involved in transmission of extracellular signals across the plasma membrane and in the cytoplasm, and/or it may also be involved in regulating some aspects of the ER functions or membrane trafficking between both membranes.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020304272ZK.pdf | 852KB | download |