The G protein α subunit (GPα1) is associated with the ER and the plasma membrane in meristematic cells of Arabidopsis and cauliflower

FEBS Letters

Huang, Hai¹  White, Elyssa¹  Ma, Hong¹  Weiss, Catherine A¹ 

[1] Cold Spring Harbor Laboratory, P.O. Box 100, Cold Spring Harbor, NY 11724, USA

关键词: Immunofluorescence;    Cell fractionation;    G protein;    Plasma membrane;    ER;    Arabidopsis;    Cauliflower;   

DOI  :  10.1016/S0014-5793(97)00378-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Towards the elucidation of the cellular function(s) of GPα1, we have characterized its subcellular localization using immunofluorescence and cell fractionation. GPα1 is not present in nuclei or chloroplasts. It is a membrane-bound protein, and analysis of isolated endoplasmic and plasma membranes indicates a good correlation between GPα1 in both the plasma membrane and the ER compartment. Interestingly, these results may suggest more different functions for GPα1: it might be involved in transmission of extracellular signals across the plasma membrane and in the cytoplasm, and/or it may also be involved in regulating some aspects of the ER functions or membrane trafficking between both membranes.

【 授权许可】

Unknown