Cytoplasmic topography of focal contacts

FEBS Letters

Cattelino, Anna¹  Dunlap, David¹  de Curtis, Ivan¹  Valtorta, Flavia¹ 

[1] DIBIT 3°A3, San Raffaele Scientific Institute, via Olgettina 58, 20132 Milan, Italy

关键词: Actin;    Cell adhesion;    Immunofluorescence;    Plasma membrane;    Scanning force microscopy;    Vinculin;   

DOI  :  10.1016/0014-5793(96)00147-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To investigate the structure of focal contacts, the cytoplasmic faces of fibroblast membranes were examined in solution by scanning force and immunofluorescence microscopy. Focal contacts were identified in scanning force topographs by correlation with fluorescence images. Finer details were resolved in topographs of the focal contacts than in fluorescence micrographs. Increased separation of ventral plasma membranes from the substrate correlated with the duration of cell culture. The cytoplasmic projections of the focal contacts also increased with the cell culture period. These changes accompanied lateral spreading of fibroblasts during the a period of several hours after seeding cells in culture medium.

【 授权许可】

Unknown