Deuterons cannot replace protons in active transport processes in yeast

FEBS Letters

Koryta, Jiří¹  Dvořáková, Milada¹  Kotyk, Amošt¹ 

[1] Department of Membrane Transport, Institute of Physiology, Czechoslovak Academy of Sciences, 142 20 Prague 4, Czechoslovakia

关键词: Heavy water;    Plasma membrane;    Proton symport;    H+-ATPase;    Yeast;   

DOI  :  10.1016/0014-5793(90)80248-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Replacement of ordinary water with heavy water causes a sharp reduction of the rates of both primary hydrogen ion transport (at the plasma membrane ATPase) and secondary symports (H⁺-associated transports of sugars and amino acids) in several species of yeast. At the same time, the hydrolytic activity of the ATPase is affected only very little. Likewise, the membrane potential, the ΔpH and, correspondingly, the accumulation ratios of the various symported solutes are altered much less. This serves as evidence that H⁺ or 11₃,0 ⁺ ions are direct participants in the various active transports of nutrients in yeast.

【 授权许可】

Unknown