Characterization of dominant lethal mutations in the yeast plasma membrane H+‐ATPase gene

FEBS Letters

Portillo, Francisco¹ 

[1] Departamento de Bioquı́mica de la Facultad de Medicina de la Universidad Autónoma de Madrid and Instituto de Investigaciones Biomédicas del Consejo Superior de Investigaciones Cientı́ficas, Arturo Duperier 4, E-28029 Madrid, Spain

关键词: H+-ATPase;    Plasma membrane;    Dominant lethal mutation;    Saccharomyces cerevisiae;   

DOI  :  10.1016/S0014-5793(96)01515-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Site-directed mutants of yeast ATPase were studied after introduction of mutant alleles into a yeast strain where these alleles were constitutively expressed and the expression of the wild-type chromosomal ATPase gene was turned off. One objection to this constitutive expression system was made apparent recently, as dominant lethal mutations are lost by gene conversion with the wild-type allele during the process. Here, the phenotypes of the mutant alleles, which were studied in a constitutive expression system, are re-evaluated under conditions in which these site-directed mutants are conditionally expressed. We show that 12 of 25 site-directed mutations previously described are actually dominant lethal alleles. In addition, we show that dominant mutant proteins interfere with transport of wild-type ATPase to the plasma membrane.

【 授权许可】

Unknown