Acylation: A new post‐translational modification specific for plasma membrane‐associated simian virus 40 large T‐antigen

FEBS Letters

Klockmann, Ulrich¹  Deppert, Wolfgang¹ 

[1] Department of Biochemistry, University of Ulm, PO Box 4066, 7900 Ulm, FRG

关键词: Simian virus 40;    Large T-antigen;    Cell fractionation;    Post-translational modification;    Protein acylation;    Membrane-association of proteins;   

DOI  :  10.1016/0014-5793(83)80081-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

SV40 transformed mouse cells (mKSA) were labeled in parallel with either [³⁵S]methionine or [³H]palmitate and subfractionated. Nuclear extracts and solubilized plasma membranes were analyzed for the presence of either ³⁵S- or ³H-labeled SV40 large tumor antigen by immunoprecipitation and SDS polyacrylamide gel electrophoresis. The majority of the [³⁵S]methionine labeled large T was recovered from the nuclear fraction, only minor amounts were detected in plasma membranes. In contrast, large T labeled specifically with [³H]palmitate was found only in the plasma membrane fraction. Our results demonstrate a specific acylation of large T associated with plasma membranes, suggesting that the membrane location of this predominantly nuclear protein is specific.

【 授权许可】

Unknown