Plasma membrane content of insulin‐regulated glucose transporter in skeletal muscle of the male Otsuka Long‐Evans Tokushima Fatty rat, a model of non‐insulin‐dependent diabetes mellitus

FEBS Letters

Sato, Takashi¹  Toide, Kiyotaka¹  Asahi, Yoshihiko¹  Man, Zhi-Wei¹ 

[1] Third Tokushima Institute of New Drug Research, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-Cho, Tokushima 771-01, Japan

关键词: Otsuka Long-Evans Tokushima Fatty rat;    Insulin-regulated glucose transporter;    Skeletal muscle;    Non-insulin-dependent diabetes mellitus model;    OLETF;    Otsuka Long-Evans Tokushima Fatty;    GLUT4;    insulin-regulated glucose transporter;    NIDDM;    non-insulin-dependent diabetes mellitus;    LETO;    Long-Evans Tokushima Otsuka;   

DOI  :  10.1016/S0014-5793(97)00369-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The male Otsuka Long-Evans Tokushima Fatty (OLETF) rat shows insulin resistance in skeletal muscle and visceral obesity. To obtain information on the mechanism of the insulin resistance in the diabetic rats, we examined the content of insulin-regulated glucose transporter (GLUT4) in skeletal muscles. The results indicate that the total content of the transporter is significantly decreased (P<0.05) in muscles of the diabetic rats. Plasma membrane content of the GLUT4 protein in muscles of the diabetic rats was increased in the basal state as compared to control rats. Hyperinsulinemic clamps increased GLUT4 levels in the plasma membrane of control rats but failed to do so in the diabetic rats. The distribution of GLUT4 in OLETF rat is reminiscent of the characteristics of human non-insulin-dependent diabetes mellitus.

【 授权许可】

Unknown