The effect of neuropeptides kyotorphin and neokyotorphin on proliferation of cultured brown preadipocytes

FEBS Letters

Zhang, Shi-Jin¹  Zinchenko, Valery²  Bronnikov, Gennady¹  Dolgacheva, Ludmila²  Kramarova, Ludmila¹  Galitovskaya, Elena² 

[1] The Wenner-Gren Institute, The Arrhenius Laboratories F3, Stockholm University, S-10691 Stockholm, Sweden;Institute of Cell Biophysics, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russia

关键词: Kyotorphin;    Neokyotorphin;    Cell proliferation;    Brown adipocyte (neuropeptides;    norepinephrine);    CGP-12177;    4-[3-[(1;    1-dimethylethyl)amino]-2-hydroxypropoxyl[-1;    3-dihydro-2H-benzimidazol-2-one hydrochloride;    IP3;    d-myo-inositol-1;    4;    5-triphosphate;    NE;    norepinephrine;   

DOI  :  10.1016/S0014-5793(97)00298-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stimulation of DNA and protein synthesis in brown preadipocytes by 1 μM neokyotorphin in serum-containing media was comparable with the effect of 1 μM norepinephrine. In serum-free medium a decrease and a shift of the maximal effect to lower concentration of neokyotorphin were observed. Kyotorphin had no effect on cell proliferation in either medium; however, 0.01–1 μM kyotorphin inhibited the cell proliferation stimulated by 1 μM norepinephrine. Norepinephrine and both peptides stimulated comparable Ca²⁺ rise in freshly isolated brown preadipocytes. The effects of neokyotorphin and norepinephrine were additive, whereas 0.03–0.3 μM kyotorphin blocked the action of 3 μM norepinephrine. The peptides did not affect the cAMP level in non-stimulated or norepinephrine-stimulated cultured cells. The effects of the peptides on the brown fat cell cultures indicate that peripheral tissue cells contain receptors for these neuropeptides.

【 授权许可】

Unknown