Three different GB virus C/hepatitis G virus genotypes

FEBS Letters

Oyunsuren, Tsendsuren³  Hikiji, Kazumasa⁵  Rezieg, Mohammed⁴  Al-Ahdal, Mohammed N.⁴  Iino, Shiro²  Kim, Boo-Sung⁶  Ohba, Ken-ichi⁷  Mizokami, Masashi⁷  Nakano, Tatsunori⁷  Park, Young-Min⁶  Lau, Johnson Y.N.⁸  Mukaide, Motokazu⁷  Lahat, Nitza¹  Shapiro, Sarah¹  Ueda, Ryuzo⁷  Orito, Etsuro⁷ 

[1] Department of Immunology, Lady Davis Carmel Hospital, Haifa, Israel;Institute of Medical Science, St. Marianna University, Kawasaki, Japan;Institute of Biotechnology, Academy of Science, Ulaan Baatar, Mongolia;King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia;Center for Molecular Biology and Cytogenetics, SRL, Inc., Tokyo, Japan;Department of Medicine, Catholic University Medical College, Seoul, South Korea;Second Department of Medicine, Nagoya City University Medical School, Nagoya, Japan;Section of Hepatobiliary Diseases, University of Florida, Gainesville, USA

关键词: Hepatitis G virus;    GB virus-C;    Genotype;    5′-UTR;    Phylogenetic tree;    Restriction fragment length polymorphism;    Reverse-transcription polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(97)00136-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 5′-untranslated region (5′-UTR) sequences of 33 GB virus C/hepatitis G virus (GBV-C/HGV) obtained from different geographic areas were determined through reverse-transcription polymerase chain reaction and dideoxy chain termination sequencing, the alignment of sequences, the estimation of the number of nucleotide substitution per site, and construction of phylogenetic trees. The 5′-UTR of GBV-HGV was found to be heterogeneous, with 70.9–99.5% homology. Three distinct phylogenetic branches were observed consistently in all phylogenetic trees. GBV-C is the prototype for one, HGV for another, and there is a new branch which consisted of GBV-C/HGV isolates from Asia. Genotype-specific restriction sites for the restriction enzymes, ScrFI and BsmFI, were identified, and a simple restriction fragment polymorphism analysis was developed for genotyping. These data provide evidence that GBV-C/HGV consists of three different genotypes. Our simple genotyping assay will also provide a tool for epidemiological studies of GBV-C/HGV infection.

【 授权许可】

Unknown