In fiscal year 2001, nonlethal fin tissues were collected for genetic analysis from fourteen stream trout populations (nine cutthroat populations and five rainbow populations) residing in headwater reaches of Wenatchee, Entiat, Lake Chelan, and Methow River tributaries. Four of these collections were made collaboratively with the U.S. Forest Service; we provided photography and they assumed responsibility for the genetic analysis. Using a portable aquarium, we photographed representative specimens of each population for a color catalog of appearance phenotypes. Analysis of paired interspersed nuclear DNA elements (PINEs) was used to characterize each population as to subspecies and level of hybridization, and a genetic purity rating was assigned to each using a modification of the Binns system originally developed in Wyoming to gauge the genetic purity of interior cutthroat trout populations.