Anaphylatoxin C5a receptor mRNA is strongly expressed in Kupffer and stellate cells and weakly in sinusoidal endothelial cells but not in hepatocytes of normal rat liver

FEBS Letters

Rothermel, Ellen¹  Jungermann, Kurt²  Timmermann, Angela²  Götze, Otto¹  Schieferdecker, Henrike L² 

[1]Abteilung für Immunologie, Georg-August-Universität Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany

[2]Institut für Biochemie und Molekulare Zellbiologie, Georg-August-Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany

关键词: Anaphylatoxin C5a receptor;    Rat liver;    Non-parenchymal liver cell;    Hepatocyte;    Quantitative RT-PCR;    r/hC5aR;    rat/human anaphylatoxin C5a receptor;    rrC5a;    recombinant rat C5a;    NPC;    non-parenchymal cell(s);    HC;    hepatocyte(s);    KC;    Kupffer cell(s);    HSC;    hepatic stellate cell(s);    SEC;    sinusoidal endothelial cell(s);    RT;    reverse transcriptase;   

DOI  :  10.1016/S0014-5793(97)00292-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Anaphylatoxins (C5a and C3a), which are generated during complement activation, have recently been shown to increase glucose output from hepatocytes (HC) in perfused rat liver. They did not act directly on HC but indirectly by prostanoid release from non-parenchymal cells (NPC), probably Kupffer cells (KC). In order to corroborate this mechanism, the distribution of anaphylatoxin receptors in the different cell types of rat liver was determined by quantitative RT-PCR with primers specific for the rat C5a receptor (rC5aR) using RNA isolated from KC, sinusoidal endothelial cells (SEC), hepatic stellate cells (HSC) and HC. In line with functional data, C5aR mRNA was detected in freshly isolated NPC but not in HC of rat liver. Mainly KC but also HSC clearly expressed C5aR mRNA, while SEC did so only weakly. KC expressed up to 10-fold more C5aR mRNA than HSC and these in turn up to 10-fold more than SEC. These results support the proposed indirect action of anaphylatoxins on HC.

【 授权许可】

Unknown