The involvement of fatty acid binding protein in peroxisomal fatty acid oxidation

FEBS Letters

Brückwilder, M.L.P.¹  Monnens, L.A.H.¹  Veerkamp, J.H.²  Trijbels, J.M.F.¹  Reubsaet, F.A.G.¹ 

[1] Department of Pediatrics, University of Nijmegen, POB 9101, 6500 HB Nijmegen, The Netherlands;Department of Biochemistry, University of Nijmegen, POB 9101, 6500 HB Nijmegen, The Netherlands

关键词: Peroxisome;    Fatty acid-binding protein;    Acyl-CoA translocation;    β-Oxidation;    Acyl-CoA oxidase;    Rat liver;   

DOI  :  10.1016/0014-5793(90)80931-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat liver fatty acid-binding protein (FABP) can function as a fatty acid donor protein for both peroxisomal and mitochondrial fatty acid oxidation, since ¹⁴C-labeled palmitic acid bound to FABP is oxidized by both organelles. FABP is, however, not detected in peroxisomes and mitochondria of rat liver by ELISA. Acyl-CoA oxidase activity of isolated peroxisomes was not changed by addition of FABP or flavaspidic acid, an inhibitor of fatty acid binding to FABP, nor by disruption of the peroxisomal membranes. These data indicate that FABP may transfer fatty acids to peroxisomes, but is not involved in the transport of acyl-CoA through the peroxisomal membrane.

【 授权许可】

Unknown