Direct evidence for the deoxyribonuclease activity of the plant ribosome inactivating protein gelonin

FEBS Letters

Beggs, Joseph M.¹  Nicolas, Emmanuelle¹  Haltiwanger, Brett M.¹  Taraschi, Theodore F.¹ 

[1] Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, 1020 Locust Street, Philadelphia, PA 19107, USA

关键词: Gelonin;    Nuclease;    Single-stranded DNA;    Zinc;   

DOI  :  10.1016/S0014-5793(97)00267-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Several plant ribotoxins, including gelonin, were reported to have additional weak nuclease activities on supercoiled DNA. The potential contribution of this activity to their cytotoxicity has not been given serious consideration due to concerns about contaminating nucleases in the protein preparations. We now report the degradation of single-stranded DNA by preparations of native plant gelonin and recombinant gelonin produced in E. coli. The DNase activity of both preparations is similarly modulated by zinc. An SDS-PAGE DNase assay identifies gelonin as the polypeptide responsible for deoxyribonuclease activity.

【 授权许可】

Unknown