DNA‐nuclease activity of the single‐chain ribosome‐inactivating proteins dianthin 30, saporin 6 and gelonin

FEBS Letters

Gasperi-Campani, A.¹  Roncuzzi, L.¹ 

[1] Department of Experimental Pathology, Via San Giacomo 14, University of Bologna, 40126 Bologna, Italy

关键词: Ribosome-inactivating protein;    Saporin 6;    Dianthin 30;    Gelonin;    DNAse activity;    Supercoiled DNA;   

DOI  :  10.1016/0014-5793(96)00776-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The single-chain ribosome-inactivating proteins (sc-RIPs) from plant origin are antiviral and antiproliferative agents employed in the preparation of immunotoxins. Similarly to the A-chains of ricin, sc-RIPs act as rRNA N-glycosidases. We demonstrate here that dianthin 30, saporin 6 and gelonin exert a specific nuclease activity on supercoiled DNA. Four specific sites of cleavage introduced by dianthin 30 and by saporin 6 and two specific sites of cleavage introduced by gelonin have been identified and mapped in pBR322.

【 授权许可】

Unknown