期刊论文详细信息
FEBS Letters | |
DNA‐nuclease activity of the single‐chain ribosome‐inactivating proteins dianthin 30, saporin 6 and gelonin | |
Gasperi-Campani, A.1  Roncuzzi, L.1  | |
[1] Department of Experimental Pathology, Via San Giacomo 14, University of Bologna, 40126 Bologna, Italy | |
关键词: Ribosome-inactivating protein; Saporin 6; Dianthin 30; Gelonin; DNAse activity; Supercoiled DNA; | |
DOI : 10.1016/0014-5793(96)00776-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The single-chain ribosome-inactivating proteins (sc-RIPs) from plant origin are antiviral and antiproliferative agents employed in the preparation of immunotoxins. Similarly to the A-chains of ricin, sc-RIPs act as rRNA N-glycosidases. We demonstrate here that dianthin 30, saporin 6 and gelonin exert a specific nuclease activity on supercoiled DNA. Four specific sites of cleavage introduced by dianthin 30 and by saporin 6 and two specific sites of cleavage introduced by gelonin have been identified and mapped in pBR322.
【 授权许可】
Unknown
【 预 览 】
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RO201912020303113ZK.pdf | 556KB | download |