Distribution of sequence‐dependent curvature in genomic DNA sequences

FEBS Letters

Gabrielian, Andrei¹  Vlahovicek, Kristian¹  Pongor, Sándor¹ 

[1] International Centre for Genetic Engineering and Biotechnology (ICGEB), Area Science Park, Padriciano 99, 34012 Trieste, Italy

关键词: DNA structure;    DNA curvature;    Genome analysis;   

DOI  :  10.1016/S0014-5793(97)00236-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The distribution of inherent, sequence-dependent curvature was calculated for a number of prokaryotic (M. genitalium, H. influenzae, M. jannaschii), viral (adenovirus 2, equine herpes virus 1), phage (M13, lambda), eukaryotic (S. cerevisiae) and mitochondrial genomes as well as E. coli and human genomic fragments. The genomic averages are in the range of 6–8°/helical turn and only about 20% of DNA is curved less than 3°/helical turn. The prokaryotes and phages appear to have a consistently higher frequency of curved DNA in their genomes than the other genomes tested. Long, highly curved segments, similar to artificially designed curved DNA, are apparently absent from the genomes. Short, curved segments, differing in G+C content may provide environmentally modulated conformational signals for gene regulation. A WWW-server was constructed for the prediction of curved sites from DNA sequences 〈http://icgeb.trieste.it/dna/curve_it.html/〉.

【 授权许可】

Unknown