The telomeric sequence is directly attached to the HRS60 subtelomeric tandem repeat in tobacco chromosomes

FEBS Letters

Fajkus, Jiří¹  Kovařík, Aleš¹  Fajkusová, Lenka²  Královics, Robert¹ 

[1] Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic;Pediatric Research Institute, Černopolní 9, 662 62 Brno, Czech Republic

关键词: DNA sequence;    Telomere;    Subtelomeric repeat;    Nicotiana tabacum;    Chromatin;    DNA curvature;    PCR;    polymerase chain reaction;    PFGE;    pulsed field gel electrophoresis;   

DOI  :  10.1016/0014-5793(95)00347-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

PCR and primers derived from the telomeric repeat (CCCTAAA)_n and from the tobacco subtelomeric tandemly repetitive sequence HRS60 (EMBL X12489) were used to amplify the region linking the two loci. A 131 bp PCR product was obtained both from total tobacco DNA and from the DNA fraction enriched for telomeres. Its sequence only consists of the telomeric primer and the attached region of the HRS60 repetitive unit up to the end of the sequence complementary to the HRS60 primer. The site of direct continuity between the two sequences is formed by a (dA)₇ tract.

【 授权许可】

Unknown