Genome-wide survey indicates involvement of loci on canine chromosomes 7 and 31 in patellar luxation in flat-coated retrievers

BMC Genetics

Herman A W Hazewinkel⁴  Henri C M Heuven²  Edwin Cuppen¹  Isaac J Nijman¹  Freek J Meutstege⁶  Gert J Breur⁷  Monique Schwencke⁵  Frank G van Steenbeek⁴  Chalika Wangdee³  Peter A J Leegwater⁴  Ineke C M Lavrijsen⁴ 

[1]Hubrecht Institute, The Royal Dutch Academy of Arts and Sciences, University Medical Center Utrecht, Utrecht, The Netherlands

[2]Animal Breeding and Genomics Centre, Wageningen University, Wageningen, The Netherlands

[3]Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand

[4]Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

[5]Dierenkliniek Putten, Roosendaalseweg 162c, Putten, The Netherlands

[6]Steenen Camer 78, Bilthoven, The Netherlands

[7]Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA

关键词: DNA sequence;    Association analysis;    Genome;    Canis;    Dog;    Knee;    Patellar luxation;   

Others  :  866249
DOI  :  10.1186/1471-2156-15-64

 received in 2013-12-09, accepted in 2014-05-14,  发布年份 2014

【 摘 要 】

Background

Patellar luxation is an orthopedic disorder in which the patella moves out of its normal location within the femoral trochlea of the knee and it can lead to osteoarthritis, lameness, and pain. In dogs it is a heritable trait, with both environmental and genetic factors contributing to the phenotype. The prevalence of patellar luxation in the Dutch Flat-Coated Retriever population is 24%. In this study, we investigated the molecular genetics of the disorder in this population.

Results

Genome-wide association analysis of 15,823 single nucleotide polymorphisms (SNPs) in 45 cases and 40 controls revealed that patellar luxation was significantly associated with a region on chromosome CFA07, and possibly with regions on CFA03, CFA31, and CFA36. The exons of the genes in these regions, 0.5 Mb combined, were analyzed further. These exons from 15 cases and a pooled sample from 15 controls were enriched using custom genomic hybridization arrays and analyzed by massive parallel DNA sequencing. In total 7257 variations were detected. Subsequently, a selection of 144 of these SNPs were genotyped in 95 Flat-Coated Retrievers. Nine SNPs, in eight genes on CFA07 and CFA31, were associated with patellar luxation (P <10^-4). Genotyping of these SNPs in samples from a variety of breeds revealed that the disease-associated allele of one synonymous SNP in a pseudogene of FMO6 was unique to Flat-Coated Retrievers.

Conclusion

Genome-wide association analysis followed by targeted DNA sequencing identified loci on chromosomes 7 and 31 as being involved in patellar luxation in the Flat-Coated Retriever breed.

【 授权许可】

   
2014 Lavrijsen et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 5.

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