Identification of Raf‐1 Ser621 kinase activity from NIH 3T3 cells as AMP‐activated protein kinase

FEBS Letters

Hardie, D.Grahame¹  Sturgill, Thomas W²  Carling, David³  Davies, Stephen P¹  Sprenkle, Amy B² 

[1] Department of Biochemistry, The University of Dundee, Dundee, DD1 4HN, UK;Howard Hughes Medical Institute, Departments of Medicine and Pharmacology, University of Virginia, Charlottesville, VA 22908, USA;Department of Molecular Medicine, Royal Postgraduate Medical School, MRC Center for Clinical Science, London, UK

关键词: Raf-1;    AMP-activated protein kinase;    cAMP-dependent protein kinase;    14-3-3 protein;    AMPK;    AMP-activated protein kinase;    MAP;    mitogen-activated protein;    PKA;    cAMP-dependent protein kinase;    GST;    glutathione S-transferase;    DMEM;    Dulbecco's modified Eagle's medium;    MCLR;    microcystin-LR;    DTT;    dithiothreitol;    2-ME;    2-mercaptoethanol;    HVE;    high-voltage electrophoresis;    FSBA;    p-fluorosulfonylbenzoyl 5′-adenosine;   

DOI  :  10.1016/S0014-5793(97)00062-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

© Federation of European Biochemical Societies.

【 授权许可】

Unknown