Regulation of maltose utilization in Saccharomyces cerevisiae by genes of the RAS/protein kinase A pathway

FEBS Letters

Tortora, Paolo³  Vanoni, Marco²  Vavassori, Monica²  Wanke, Valeria²  Thevelein, Johan M¹ 

[1] Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Leuven, Belgium;Dipartimento di Fisiologia e Biochimica Generali, Sezione di Biochimica Comparata, Università degli Studi di Milano, via Celoria 26, I-20133 Milano, Italy;Dipartimento di Fisiologia e Biochimica Generali, Sezione di Chimica Biologica, Università degli Studi di Milano, Milano, Italy

关键词: Catabolite inactivation;    Catabolite repression;    Gene expression;    Maltase;    Maltose permease;   

DOI  :  10.1016/S0014-5793(97)00009-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Saccharomyces cerevisiae maltose utilization requires a functional MAL locus, each composed of three genes: MALR (gene 3) encoding a regulatory protein, MALT (gene 1) encoding maltose permease and MALS (gene 2) encoding maltase. We show that constitutive activation of the RAS/protein kinase A pathway severely reduces growth of MAL1 strains on maltose. This may be a consequence of reduction in MALT mRNA, reduced V _max and increased catabolite inactivation of the MALT-encoded maltose transporter in the MAL1 strain. Mutations in the GGS1/TPS1 gene, which restricts glucose influx and possibly affects signalling, relieve carbon catabolite repression on both maltase and maltose permease and reduce maltose permease inactivation.

【 授权许可】

Unknown