Regulation of yeast fructose‐1,6‐bisphosphatase in strains containing multicopy plasmids coding for this enzyme

FEBS Letters

de la Guerra, R.¹  Valdés-Hevia, M.D.¹  Gancedo, J.M.¹ 

[1] Instituto de Investigaciones Biomédicas del CSIC, Facultad de Medicina, UAM, Arzobispo Morcillo 4, E-28029 Madrid, Spain

关键词: Fructose-1;    6-bisphosphatase;    Catabolite inactivation;    Catabolite repression;    Gene expression;    (Saccharomyces cerevisiae);   

DOI  :  10.1016/0014-5793(88)81004-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The structural gene for yeast fructose-1,6-bisphosphatase (FbPase) has been cloned by complementation of a strain carrying the fbp1-1 mutation and lacking FbPase activity. By Northern analysis it could be shown that the fbp1-1 mutation does not interfere with the transcription of the gene although no normal active enzyme is produced. Strains transformed with the cloned gene on a multicopy plasmid overproduce FbPase but are still subject to strong catabolite repression. Catabolite inactivation, however, is slowed down in such strains.

【 授权许可】

Unknown