Tissue‐specific subunit of the mouse cytosolic chaperonin‐containing TCP‐1

FEBS Letters

Willison, Keith R²  Kubota, Hiroshi²  Kerr, Shona M¹  Hynes, Gillian M² 

[1] Medical Research Council Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK;Cancer Research Campaign Centre for Cell and Molecular Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK

关键词: Molecular chaperone;    CCT;    Mouse testis;    Anti-peptide antibody;    Tissue-specific subunit;    CCT;    chaperonin containing TCP-1;    Ccta;    Cctb;    Cctg;    Cctd;    Ccte;    Cctz-1;    Cctz-2;    Ccth and Cctq;    genes encoding α;    β;    γ;    δ;    ϵ;    ζ-1;    ζ-2;    η and θ subunits of CCT;    respectively;    TCP-1;    t-complex polypeptide 1;    pI;    isoelectric point;    2-D PAGE;    2-dimensional polyacrylamide gel electrophoresis;    Hsp70;    70 kDa heat shock protein;    Hsp60;    60 kDa heat shock protein;   

DOI  :  10.1016/S0014-5793(96)01501-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned a novel Tcp-1-related mouse testis cDNA encoding a polypeptide of 531 amino acids which shares 81.2% identity with the ζ subunit of the mouse cytosolic chaperonin-containing TCP-1 (CCT). Immunoblot analysis of mouse testis CCT subunits separated by 2-dimensional gel electrophoresis indicates that this novel gene, Cctz-2, encodes a CCT subunit of M _r 57 000 and pI 7.1. Cctz-2 mRNA is detected only in testis whereas the other Cctz gene, Cctz-1, is expressed in all tissues investigated. The CCTζ-2 subunit may have specific functions in the folding of testicular proteins and for interactions with testicular molecular chaperones.

【 授权许可】

Unknown