Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in adult mouse testis

FEBS Letters

Takamatsu, Nobuhiko¹  Yamashita, Atsushi¹  Ito, Michihiko¹  Shiba, Tadayoshi¹ 

[1] Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228 Japan

关键词: Sox;    Leucine zipper;    HMG box;    Ca2+/calmodulin-dependent protein kinase IV;    Mouse testis;    HMG;    high mobility group;    LZ;    leucine zipper;    CaMK;    Ca2+/calmodulin-dependent protein kinase;    GST;    glutathione S-transferase;    HMK;    heart muscle kinase;    RT-PCR;    reverse transcriptase-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(00)01987-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

SoxLZ/Sox6, a member of the Sox protein family, contains a leucine zipper motif in addition to an HMG box, which is its DNA binding domain. Here we have identified a novel SoxLZ/Sox6 binding protein, termed Solt, which we obtained independently using both a far-Western blot and a yeast two-hybrid screen. Like SoxLZ/Sox6 mRNA, Solt mRNA was exclusively expressed in the testis in mouse. Solt contains an unusual leucine zipper, which bound to the leucine zipper region of SoxLZ/Sox6 in vitro. In transient transfection assays in CHO cells with SoxLZ/Sox6 containing the transactivational region of herpes simplex virus VP16, expression of a reporter gene that carries a cis binding region for Sox proteins was significantly enhanced by the co-expression of Solt and Ca²⁺/calmodulin-dependent protein kinase IV.

【 授权许可】

Unknown