Antibody characterisation of two distinct conformations of the chaperonin‐containing TCP‐1 from mouse testis

FEBS Letters

Kubota, Hiroshi¹  Hynes, Gillian¹  Willison, Keith R.¹ 

[1] CRC Centre for Cell and Molecular Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK

关键词: Molecular chaperone;    CCT;    Mouse testis;    Anti-peptide antibody;    Non-denaturing IEF;    IEF;    isoelectric focusing;    PAGE;    polyacrylamide gel electrophoresis;    CCT;    chaperonin-containing TCP-1;    ECL;    enhanced chemiluminescence;    PPD;    purified protein derivative;    Fmoc;    9-fluorenylmethyloxycarbonyl;    CHAPS;    (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate);   

DOI  :  10.1016/0014-5793(94)01408-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe a panel of antibodies specific to individual subunits of the chaperonin-containing TCP-1 (CCT) and one antibody that reacts with all the subunits of CCT. Immunoblot analysis of CCT purified from mouse testis suggests that the testis-specific subunit, S6, may be related to CCTζ and that a co-purifying 63 kDa protein may be a novel subunit of CCT. Using these antibodies in the analysis of CCT subjected to non-denaturing IEF we observed the resolution of two distinct conformations of CCT, which differ in their susceptibility to proteolysis and in the number of associated polypeptides.

【 授权许可】

Unknown