Solubility diagram of the Rhodobacter sphaeroides reaction center as a function of PEG concentration

FEBS Letters

Gaucher, Jean-François¹  Riès-Kautt, Madeleine¹  Reiss-Husson, Françoise²  Ducruix, Arnaud¹ 

[1] Laboratoire d'Enzymologie et de Biochimie Structurales, CNRS, Bât. 34, 91198 Gif-sur-Yvette, France;Centre de Génétique Moléculaire, CNRS, 91198 Gif-sur-Yvette, France

关键词: β-Octylglucoside;    Crystallization;    Solubility;    Membrane protein;    Photochemical reaction center;    Polyethylene glycol;    RC;    photochemical reaction center;    β-OG;    β-d-octylglucoside;    LDAO;    lauryldimethylamine N-oxide;    Rb;    Rhodobacter;    PEG;    polyethylene glycol 4000;    C;    conductivity;    n D 18°C;    refractive index at 18°C;   

DOI  :  10.1016/S0014-5793(96)01446-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to quantify the effect of polyethylene glycol 4000 (PEG) on the solubility of an integral membrane protein, we have crystallized the photochemical reaction center from Rhodobacter sphaeroides Y by batch method on a large range of PEG. The measurement of the solubility diagram display a semi-logarithmic dependence of solubility versus PEG concentration. Comparison of our results with previously published ones [Odahara, T., Ataka, M. and Katsura, M. (1994) Acta Cryst. D50, 639–642] suggests a notable effect of additional 1,2,3-heptane-triol and/or temperature on photochemical reaction center solubility.

【 授权许可】

Unknown