Aqueous micellar systems in membrane protein crystallization

FEBS Letters

Weckström, Kristian¹ 

[1] European Molecular Biology Laboratory, 156X, 38042 Grenoble Cedex, France

关键词: Detergent;    Crystallization;    Membrane protein;    Polyethylene glycol;   

DOI  :  10.1016/0014-5793(85)80111-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of NaCl and polyethylene glycol (PEG) 4000 on the lower consolution boundary (LCB) of a nonionic surfactant (C₈E₅) were studied and compared. Micellar systems where NaCI and PEG 4000 are present are often used in membrane protein crystallization. While sodium chloride shifts the surfactant LCB to lower temperatures without a significant change in the shape of the boundary, PEG produces a large solubility change strongly depending on the surfactant concentration. The salt effect is explained by a reduced interaction of the micellar oligooxyethylene chains with the water and the PEG effect by an unfavourable configurational interaction between the C₈E₅ micelles and PEG molecules.

【 授权许可】

Unknown