Crystallization of reaction center I of photosynthesis Low‐concentration crystallization of photoactive protein complexes from the cyanobacterium Synechococcus sp

FEBS Letters

Gerken, S.¹  Witt, I.²  Saenger, W.²  Dekker, J.P.¹  Rögner, M.¹  Witt, H.T.¹ 

[1] >Max-Volmer-Institut für Biophysikalische und Physikalische Chemie, Technische Universität Berlin, Strasse des 17. Juni 135, 1000 Berlin 12 Germany;Institut für Kristallographie, Freie Universität Berlin, Takustr. 6, 1000 Berlin 33, Germany

关键词: Photosystem I;    Reaction center;    Membrane protein;    Crystallization;    (Synechococcus sp.);    APC;    allophycocyanin;    asc;    ascorbate;    β-DM;    β-dodecyl-D-maltoside;    BV;    benzyl viologen;    Car;    carotenoid;    Chl;    chlorophyll;    PEG;    polyethylene glycol;    PMS;    phenazine methosulfate;    PS;    photosystem;    SB;    sulfobetain;   

DOI  :  10.1016/0014-5793(87)80937-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The integral membrane protein complex of the photosystem I reaction center from the cyanobacterium Synechococcus sp. has been isolated and highly purified. At extremely low protein complex concentrations (50 μg/ml), crystallization occurred. The crystals are dichroic, photoactive and show an X-ray powder diffraction pattern.

【 授权许可】

Unknown