Structural studies on improved crystals of the photosystem I reaction centre from Phormidium laminosum

FEBS Letters

Ford, Robert C.²  Pauptit, Richard A.³  Holzenburg, Andreas¹ 

[1] Maurice E. Müller Institute for High Resolution Electron Microscopy, Biocentre, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland;Department of Biophysical Chemistry, Biocentre, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland;Department of Structural Biology, Biocentre, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland

关键词: Membrane protein;    Crystallization;    Photosystem I;    Reaction center;    Chl;    chlorophyll;    PS;    photosystem;   

DOI  :  10.1016/0014-5793(88)80517-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Improved crystallization methods led to the growth of large single crystals of the photosystem I reaction centre isolated from the thermophilic cyanobacterium Phormidium laminosum. Although the crystals exhibited a high degree of mosaicity, some preliminary X-ray diffraction studies were performed. The highest order reflections found correspond to interplanar spacings of approx. 0.8 nm. In conjunction with electron microscopy of thin three-dimensional crystals, a triclinic or monoclinic crystal system with unit cell dimensions 30 × 18 × 18 nm was assigned. In addition, our data suggest the presence of four trimeric complexes per unit cell, which may be organised as two face-to-face pairs.

【 授权许可】

Unknown