Preliminary X‐ray crystallographic studies of photosynthetic reaction center from a thermophilic sulfur bacterium, Chromatium tepidum

FEBS Letters

Inaka, Koji²  Nozawa, Tsunenori¹  Kobayashi, Masayuki¹  Katayama, Naoko²  Motojima, Fumihiro²  Miki, Kunio² 

[1] Department of Biochemistry and Engineering, Faculty of Engineering, Tohoku University, Aramaki, Aoba, Sendai 980-77, Japan;Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 227, Japan

关键词: Membrane protein;    Reaction center;    Photosynthesis;    Crystallization;    X-ray structure analysis;   

DOI  :  10.1016/0014-5793(94)00534-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A membrane protein complex, photosynthetic reaction center purified from the thermophilic purple sulfur bacterium, Chromatium tepidum has been crystallized from a phosphate butter containing a detergent, n-octyl-β-D-glucopyranoside and a precipitant, polyethylene glycol 4000. The crystals diffracted X-rays beyond 3Å resolution with synchrotron radiation and are suitable for high-resolution X-ray crystallographic studies. The crystals belong to the orthorhombic space group P2₁2₁2₁ with unit-cell dimensions of a = 136Å, b = 197Å, and c = 82Å. Assuming that they contain one reaction center complex in the asymmetric unit, V _M was calculated to be 4.3 ų/Da, which agrees with the values obtained in the membrane protein complexes.

【 授权许可】

Unknown