Complementation of a yeast Δpkc1 mutant by the Arabidopsis protein ANT

FEBS Letters

Morandini, Piero¹  Vergani, Paola¹  Soave, Carlo¹ 

[1]Department of Biology, Section of Plant Physiology and Biochemistry, University of Milano, Via Celoria, 26, 20133 Milano, Italy

关键词: Functional complementation;    Plant;    Protein kinase C;    Signal transduction;    Transcription factor;    Yeast;   

DOI  :  10.1016/S0014-5793(96)01399-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Saccharomyces cerevisiae protein kinase C homologue, PKC1, is involved in maintenance of cell integrity during polarized growth. We have used a mutant complementation approach to investigate related signal transduction pathways in higher plants. Here we report the isolation of a cDNA from Arabidopsis thaliana which partially suppresses the lytic defect of a Δpkc1 yeast strain. The encoded protein, ANT, belongs to the AP2-related gene family and is essential for ovule development. Expression in yeast of a LexA-ANT fusion protein activates transcription of a reporter gene from promoters containing lexA operators. Our results support the idea that ANT acts as transcriptional activator in planta.

【 授权许可】

Unknown