Co‐elevation of brain natriuretic peptide and proprotein‐processing endoprotease furin after myocardial infarction in rats

FEBS Letters

Sawada, Yoshie²  Tanaka, Shigeyasu¹  Minamino, Naoto³  Kanda, Tsugiyasu⁵  Sakamaki, Tetsuo⁴  Nagai, Ryozo⁴  Inoue, Masahiro⁴  Takeuchi, Toshiyuki² 

[1] Department of Cell Biology, The Institute for Molecular and Cellular Regulation, Gunma University School of Medicine, Maebashi 371, Japan;Department of Molecular Medicine, Gunma University School of Medicine, Maebashi 371, Japan;National Cardiovascular Center Research Institute, Suita, Osaka 565, Japan;the Second Department of Internal Medicine, Gunma University School of Medicine, Maebashi 371, Japan;Department of Laboratory Medicine, Gunma University School of Medicine, Maebashi 371, Japan

关键词: Brain natriuretic peptide;    Furin;    PACE4;    Processing;    Myocardial infarction;   

DOI  :  10.1016/S0014-5793(96)01385-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the expression of the yeast Kex2 family endoproteases furin and PACE4, and brain natriuretic peptide (BNP) in the atrium and ventricle after infarction as well as the conversion of the BNP precursor γBNP to BNP-45. In a rat heart failure model, plasma BNP rose in two phases – first at day 3, and again at day 14. BNP mRNA, as measured by Northern blot analysis, increased strongly at day 3, then at days 14 and 28 less strongly in the atrium, and in the ventricle it increased weakly at day 3, then strongly at days 14 and 28. Furin mRNA showed the same pattern of expression as that of BNP message, whereas PACE4 message stayed unchanged after the infarction. Both furin and BNP were immunostained in the myocardium adjacent to the infarcted tissue. We suggest that after myocardial infarction, furin is co-expressed with BNP in both the atrium and ventricle, and that furin may be responsible for the conversion of γBNP to BNP-45.

【 授权许可】

Unknown