Cellular processing of the neurotrophin precursors of NT3 and BDNF by the mammalian proprotein convertases

FEBS Letters

Chrétien, Michel²  Pareek, Sangeeta¹  Benjannet, Suzanne²  Murphy, Richard A.¹  Seidah, Nabil G.³ 

[1] Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, 3801 University, Montreal, Que. H3A 2B4, Canada;J.A. DeSève Laboratories of Molecular Neuroendocrinology, Clinical Research Institute of Montreal, University of Montreal, 110 Pine Avenue West, Montreal, Que. H2 W 1R7, Canada;J.A. DeSève Laboratories of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, University of Montreal, 110 Pine Avenue West, Montreal, Que. H2 W 1R7, Canada

关键词: BDNF;    NT3;    Convertase;    Furin;    PACE4;    PC5/6-B;    NGF;    nerve growth factor;    BDNF;    brain-derived neurotrophic factor;    NT3;    neurotrophin-3;    VV;    vaccinia virus;   

DOI  :  10.1016/0014-5793(95)01520-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to define the enzymes responsible for the maturation of the precursors of brain-derived neurotrophic factor (proBDNF) and neurotrophin-3 (proNT3), we have analysed their biosynthesis and intracellular processing by the proprotein convertases furin, PC1, PC2, PACE4, PC5 and its isoform PC5/6-B. In these studies, we utilized a vaccinia virus expression system in either BSC40 or the furin activity-deficient LoVo cells. Results demonstrated that in both cells furin and, to a lesser extent, PACE4 and PC5/6-B effectively process proBDNF and proNT3. Furthermore, we have determined that human proNT3 is sulfated, suggesting that processing of proNT3 occurs following the arrival of the precursor to the Trans Golgi Network.

【 授权许可】

Unknown