Effect of side groups on the action of β‐xylosidase from Trichoderma reesei against substituted xylo‐oligosaccharides

FEBS Letters

Luonteri, Elina²  Teleman, Anita¹  Tenkanen, Maija² 

[1] VTT Chemical Technology, P.O. Box 1401, FIN-02044 VTT, Finland;VTT Biotechnology and Food Research, P.O. Box 1501, FIN-02044 VTT, Finland

关键词: Trichoderma reesei β-xylosidase;    Hydrolysis;    Substituted xylo-oligosaccharide;    High-performance anion-exchange chromatography;    NMR;   

DOI  :  10.1016/S0014-5793(96)01313-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The action of β-xylosidase from Trichoderma reesei against different substituted xylo-oligosaccharides was studied. The enzyme cleaved off all unsubstituted xylose units from the non-reducing end of 1,2-linked uronic acid substituted xylo-oligosaccharides. Surprisingly, an l-arabinofuranosyl group linked α-1,3 to the xylopyranosyl ring was found to protect the β-1,4-xylosidic linkage before the substituted xylose unit from being cleaved by the β-xylosidase. Most probably the 1,3-linked substituent sterically hinders the hydrolysis. According to the results of the present work, β-xylosidase of T. reesei is not able to remove all unsubstituted xylose units from the non-reducing end of substituted xylo-oligosaccharides, as had been believed previously.

【 授权许可】

Unknown