FEBS Letters | |
Complete amino acid sequence of the A chain of mistletoe lectin I | |
Stoeva, Stanka1  Voelter, Wolfgang1  Stiefel, Thomas2  Soler, Montserrat Huguet1  Schwamborn, Cornelia2  Wilhelm, Sabine2  | |
[1] Abteilung fur Physikalische Biochemie des Physiologisch-chemischen Instituts der Universität Tübingen, Hoppe-Seyler-Str. 4, D-72076 Tübingen, Germany;Medisculab Arzneimittel GmbH, Fellbach, Germany | |
关键词: Mistletoe lectin I; Immunomodulation; Ribosome-inactivating protein; Amino acid sequence; Viscum album; ML-I; mistletoe lectin 1; MLA; A chain of mistletoe lectin 1; MLB; B chain of mistletoe lectin I; MALDI-MS; matrix-assisted laser desorption ionization mass spectrometry; RIP; ribosome-inactivating protein; | |
DOI : 10.1016/S0014-5793(96)01309-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The complete amino acid sequence of the A chain of mistletoe lectin I was determined via Edman degradation sequencing of the N-terminus and tryptic and endoproteinase Asp-N overlapping fragments, amino acid analysis and MALDIMS. The data obtained show a great homology with the chains of ribosome-inactivating proteins such as ricin and abrin with 111 (abrin-a) and 103 (ricin-D) amino acid residues conserved, respectively. The knowledge of the primary structure of MLA will have a fundamental impact on elucidating the biological function of medically applied mistletoe lectins on a molecular basis.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020303681ZK.pdf | 597KB | download |