Complete amino acid sequence of the A chain of mistletoe lectin I

FEBS Letters

Stoeva, Stanka¹  Voelter, Wolfgang¹  Stiefel, Thomas²  Soler, Montserrat Huguet¹  Schwamborn, Cornelia²  Wilhelm, Sabine² 

[1] Abteilung fur Physikalische Biochemie des Physiologisch-chemischen Instituts der Universität Tübingen, Hoppe-Seyler-Str. 4, D-72076 Tübingen, Germany;Medisculab Arzneimittel GmbH, Fellbach, Germany

关键词: Mistletoe lectin I;    Immunomodulation;    Ribosome-inactivating protein;    Amino acid sequence;    Viscum album;    ML-I;    mistletoe lectin 1;    MLA;    A chain of mistletoe lectin 1;    MLB;    B chain of mistletoe lectin I;    MALDI-MS;    matrix-assisted laser desorption ionization mass spectrometry;    RIP;    ribosome-inactivating protein;   

DOI  :  10.1016/S0014-5793(96)01309-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequence of the A chain of mistletoe lectin I was determined via Edman degradation sequencing of the N-terminus and tryptic and endoproteinase Asp-N overlapping fragments, amino acid analysis and MALDIMS. The data obtained show a great homology with the chains of ribosome-inactivating proteins such as ricin and abrin with 111 (abrin-a) and 103 (ricin-D) amino acid residues conserved, respectively. The knowledge of the primary structure of MLA will have a fundamental impact on elucidating the biological function of medically applied mistletoe lectins on a molecular basis.

【 授权许可】

Unknown