The site of action of the A‐chain of mistletoe lectin I on eukaryotic ribosomes The RNA N‐glycosidase activity of the protein

FEBS Letters

Franz, Hartmut²  Endo, Yaeta¹  Tsurugi, Kunio¹ 

[1] Department of Biochemistry, Yamanashi Medical College, Tamaho, Nakakoma, Yamanashi 409-38, Japan;Staatliches Institut für Immunpräparate und Nährmedien, Berlin, GDR

关键词: Mistletoe lectin I;    RNA N-glycosidase;    Ribosomal action site;   

DOI  :  10.1016/0014-5793(88)80853-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The site of action of the A-chain of mistletoe lectin (ML-A) from Viscum album on eukaryotic ribosomes was studied. Treatment of rat liver ribosomes with ML-A, followed by treatment of the isolated rRNA with aniline, caused the release of a fragment with about 450 nucleotides from 28 S rRNA. Further analysis of nucleotide sequences of this fragment revealed that the aniline-sensitive site of phosphodiester bond was between positions A-4324 and G-4325 in 28 S rRNA. These results indicate that ML-A inactivates the ribosomes by cleaving a N-glycosidic bond at A-4324 of 28 S rRNA in the ribosomes as ricin A-chain does.

【 授权许可】

Unknown