Vacuolating toxin from Helicobacter pylori activates cellular signaling and pepsinogen secretion in human gastric adenocarcinoma cells

FEBS Letters

Lin, Yuh-Ling¹  Chen, Kuei-Tian¹  Chan, Err-Cheng¹ 

[1] Medical Biotechnology Laboratory, School of Medical Technology, Chang Gung College of Medicine and Technology, 259 Wen-Hua 1st Road, Taoyuan, Taiwan, ROC

关键词: Vacuolating toxin;    Helicobacter pylori;    Cellular signaling;    Pepsinogen;    Peptic ulcer;    Phosphorylation;   

DOI  :  10.1016/S0014-5793(96)01304-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated cellular signaling and pepsinogen secretion in the human gastric adenocarcinoma cell line AGS which was pretreated with the purified vacuolating cytotoxin from Helicobacter pylori. Results indicated that vacuolating toxin increased the levels of inositol phosphates, cytosolic free calcium concentration, adenosine 3′,5′-cyclic monophosphate, and phosphorylation of 31 kDa and 22 kDa proteins in the host cells. Moreover, pepsinogen secretion from AGS cells was stimulated with increasing concentrations of cytotoxin. We conclude that besides the H. pylori cytotoxin-induced cellular vacuoles, cytotoxin-stimulated signaling mediators and pepsinogen release are important factors involved in the etiology of chronic active gastritis and peptic ulcer disease.

【 授权许可】

Unknown