Arrest of mitochondrial biogenesis in copper‐treated sycamore cells

FEBS Letters

Casimiro, Adalcina¹  Aubert, Serge²  Pádula, Mário²  Bligny, Richard² 

[1] Laboratório de Fisiologia e Bioquímica Vegetais, DBV, Faculdade de Ciências da Universidade de Lisboa, Bloco C-2, Campo Grande, 1700 Lisbon, Portugal;Laboratoire de Physiologie Cellulaire Végétale, URA CNRS No. 576, DBMS, CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France

关键词: Mitochondria;    Respiration;    Copper;    Stress;    Acer pseudoplatanus;   

DOI  :  10.1016/S0014-5793(96)01260-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sycamore suspension cells (Acer pseudoplatanus L.) were grown in the presence of sublethal concentrations of copper (50 μM). During the first 5–6 days of treatment, growth was not affected, but cell respiration (coupled and uncoupled) declined to ∼60% of its normal value. This decline of respiration was attributed to a progressive diminution of the number of mitochondria in copper-treated cells, based on the demonstration of the concomitant decline of (1) cardiolipin (diphosphatidylglycerol) and cytochrome aa ₃ (cytochrome oxidase), two specific markers of mitochondrial inner membrane, and (2) fumarase activity, a specific marker of mitochondrial matrix space. In addition, the mitochondria extracted from copper-treated cells presented the same properties as those from control cells, concerning substrate oxidation, cardiolipin and cytochrome aa ₃ contents, and fumarase activity. These results strongly suggest that copper triggered an arrest of mitochondrial biogenesis, which preceded cell division arrest.

【 授权许可】

Unknown