Ectopic expression of CHOP (GADD153) induces apoptosis in M1 myeloblastic leukemia cells

FEBS Letters

Matsumoto, Makoto¹  Minami, Masashi¹  Takeda, Kiyoshi¹  Sakao, Yoshimitsu¹  Akira, Shizuo¹ 

[1] Institute for Molecular and Cellular Biology, Osaka University 1-3 Yamada-oka, Suita, Osaka 565, Japan

关键词: CHOP;    GADD153;    Apoptosis;    p53;    Leucine zipper;    Bcl-2;    CHOP;    C/EBP-homologous protein;    GADD153;    growth arrest- and DNA damage-inducible gene 153;    C/EBP;    CCAAT/enhancer-binding protein;    IPTG;    isopropyl ß-d-thiogalactoside;   

DOI  :  10.1016/0014-5793(96)01016-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CHOP (GADD153) is a member of the C/EBP family and a stress-induced protein. To investigate the role of CHOP in cellular growth, we expressed CHOP conditionally in M1 myeloblastic leukemia cells that do not express p53 protein. More than 60% of M1 cells died through apoptosis 72 h after CHOP induction. Site-directed mutagenesis revealed that this process requires leucine zipper domain but neither intact basic region nor trans-activation domain. CHOP-mediated apoptosis accompanied downregulation of bcl-2 mRNA and overexpression of Bcl-2 delayed the process. Our results indicate that CHOP can induce apoptosis in a p53-independent manner.

【 授权许可】

Unknown