SNAP‐25 is present on chromaffin granules and acts as a SNAP receptor

FEBS Letters

Genma, Tomohiro²  Kozaki, Shunji³  Yamamoto, Akitsugu¹  Tagaya, Mitsuo²  Mizushima, Shoji² 

[1] Department of Physiology, Kansai Medical University, Moriguchi, Osaka 570, Japan;School of Life Science, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192-03, Japan;Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Sakai, Osaka 593, Japan

关键词: Chromaffin granule;    N-Ethylmaleimide-sensitive factor;    Soluble NSF attachment protein;    NSF;    N-ethylmaleimide-sensitive factor;    SNAP;    soluble NSF attachment protein;    SNARE;    SNAP receptor;    MAb;    monoclonal antibody;    PBS;    phosphate-buffered saline;    BSA;    bovine serum albumin;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(96)00932-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

SNAP-25 is located on the plasma membrane and essential for exocytosis of neurotransmitters. It was suggested that SNAP-25 and syntaxin 1 via the interaction with VAMP-2 located on synaptic vesicles mediate the docking of the vesicles with the plasma membrane. In the present study, by means of biochemical and morphological analyses, we showed that SNAP-25 is present on chromaffin granules as well as on the plasma membrane. Reconstitution and immunoprecipitation analyses revealed that SNAP-25 on chromaffin granules has essentially the same properties as does SNAP-25 on the plasma membrane.

【 授权许可】

Unknown