The effects of SNAP/SNARE complexes on the ATPase of NSF

FEBS Letters

Whiteheart, Sidney W.¹  Matveeva, Elena¹ 

[1] Department of Biochemistry, Chandler Medical Center, University of Kentucky College of Medicine, 800 Rose Street, Lexington, KY 40536-0084, USA

关键词: N-Ethylmaleimide sensitive factor;    Soluble NSF attachment protein;    SNAP receptor;    Membrane fusion;    AAA ATPase;    Vesicular traffic;   

DOI  :  10.1016/S0014-5793(98)01071-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ATPase of the N-ethylmaleimide sensitive factor (NSF) appears to be central to the events that culminate in vesicle-target membrane fusion. Complexes containing different combinations of NSF, α-SNAP, Vamp-2 (synaptobrevin 2), syntaxin 1, and SNAP-25 were reconstituted and then tested for their effect on the ATPase of NSF. While NSF interacts with all α-SNAP-containing complexes, only the α-SNAP/t-SNARE complex significantly stimulated ATPase activity. This stimulation was dependent on increasing SNAP/t-SNARE complex and was saturable. The apparent stimulation of ATPase activity is due to a 10-fold increase in initial hydrolysis rate. Complex containing both v- and t-SNAREs bound significantly more α-SNAP but did not stimulate the ATPase of NSF.

【 授权许可】

Unknown