NADH‐mediated DNA damage induced by a new coenzyme, pyrroloquinoline quinone, in the presence of copper(II) ion

FEBS Letters

Kawanishi, Shosuke²  Hiraku, Yusuke¹ 

[1] Department of Public Health, Graduate School of Medicine, Kyoto University, Kyoto 606, Japan;Department of Hygiene, Mie University School of Medicine, Tsu Mie 514, Japan

关键词: DNA damage;    Pyrroloquinoline quinone;    NADH;    Active oxygen species;    Copper;    PQQ;    pyrroloquinoline quinone;    PQQ'H;    semiquinone radical of PQQ;    ESR;    electron spin resonance;    DTPA;    diethylenetriamine-N;    N;    N′;    N″;    N″-pentaacetic acid;    SOD;    superoxide dismutase;    NBT;    nitroblue tetrazolium;   

DOI  :  10.1016/0014-5793(96)00908-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pyrroloquinoline quinone (PQQ) plays a role as a vitamin or growth factor. Low concentrations of PQQ induced DNA cleavage sites frequently at thymine and cytosine residues in the presence of NADH and Cu(II). Catalase and bathocuproine inhibited DNA damage, whereas free hydroxyl radical scavengers did not. Electron spin resonance and UV-visible spectrometries showed generation of semiquinone radical and superoxide during the reaction of PQQ with NADH. These results suggest that NADH-dependent PQQ redox cycle generated superoxide and hydrogen peroxide to mediate copper-dependent DNA damage. The present study has proposed a requirement to investigate the potentiality of PQQ carcinogenicity.

【 授权许可】

Unknown