Neopterin activates transcription factor nuclear factor‐κB in vascular smooth muscle cells

FEBS Letters

Schobersberger, Wolfgang²  Pelzer, Larissa¹  Fuchs, Dietmar³  Hoffmann, Georg¹  Frede, Stilla¹  Wachter, Helmut³  Fandrey, Joachim¹  Grote, Jürgen¹ 

[1] Department of Physiology, University of Bonn, Nussallee 11, D-53115 Bonn, Germany;Clinic for Anaesthesia and General Intensive Care Medicine, University of Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria;Institute of Medical Chemistry and Biochemistry, University of Innsbruck, Fritz Pregl Strasse 3, A-6020 Innsbruck, Austria

关键词: Neopterin;    Nuclear factor-κB;    Inducible nitric oxide synthase;    Nitric oxide;   

DOI  :  10.1016/0014-5793(96)00729-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously shown that the pteridine compound neopterin stimulates inducible nitric oxide synthase (iNOS) gene expression in vascular smooth muscle cells in vitro. The mechanisms whereby neopterin exhibits these effects remained unclear. The present study demonstrates that neopterin induces the translocation of the transcription factor nuclear factor-κB (NF-κB) to the nucleus. Pretreatment of cells with the antioxidant pyrrolidine dithiocarbamate completely suppressed the effects of neopterin on NF-κB activation, iNOS gene expression, and nitric oxide release. From these data we conclude that neopterin activates the translocation of NF-κB subunits to the nucleus by modulating the intracellular redox state. This is one possible explanation for the impact of neopterin on iNOS gene expression.

【 授权许可】

Unknown