Impaired glutathione biosynthesis in the ischemic‐reperfused rabbit myocardium

FEBS Letters

Mezzetti, Andrea¹  Pierdomenico, Sante D.¹  de Gioia, Sergio¹  Lapenna, Domenico¹  Di Ilio, Carmine²  Cuccurullo, Franco¹  Ciofani, Giuliano¹ 

[1] Istituto di Fisiopatologia Medica, Universita' ‘G. D'Annunzio’, Facolta' di Medicina e Chirurgia, 66100 Chieti, Italy;Istituto di Scienze Biochimiche, Universita' ‘G. D'Annunzio’, Facolta' di Medicina e Chirurgia, 66100 Chieti, Italy

关键词: Glutathione;    Glutathione-related enzyme;    Ischemia-reperfusion;    Perfused heart;    Oxidative stress;   

DOI  :  10.1016/0014-5793(96)00705-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Non-protein thiols (NP-SH) and the activities of the glutathione status-regulating enzymes γ-glutamylcysteine synthetase (G-GCS), γ-glutamyl transpeptidase (G-GT) and glutathione reductase (GR) were assessed in perfused rabbit hearts subjected to severe (60 min) or mild (7 min) total ischemia and 30 min reperfusion. Severe ischemia significantly decreased NP-SH, which were further depressed on reperfusion together with a significant decline in G-GCS activity; G-GT and GR activities were unchanged. Specific analytes were unaffected by mild ischemia-reperfusion. Thus, impaired enzymatic biosynthesis of GSH is operative in the reperfused rabbit myocardium after 60 min ischemia. This phenomenon may favour myocardial GSH depression and oxidative reperfusion injury after severe ischemia.

【 授权许可】

Unknown