Elucidating the contribution of mitochondrial glutathione to ferroptosis in cardiomyocytes

Redox Biology

Valerian E. Kagan¹  Claudette M. St Croix²  Vladimir A. Tyurin³  Alexandr A. Kapralov³  Hülya Bayır³  Sabzali Javadov³  Yulia Y. Tyurina³  Xavier R. Chapa-Dubocq⁴  Sehwan Jang⁴ 

[1] Department of Critical Care Medicine, University of Pittsburgh, and Children's Neuroscience Institute, UPMC Children's Hospital, Pittsburgh, PA, 15260, USA;Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, 15260, USA;Department of Environmental and Occupational Health, Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA, 15260, USA;Department of Physiology, University of Puerto Rico School of Medicine, San Juan, PR, 00936- 5067, USA;

关键词: Ferroptosis;    Heart;    Mitochondria;    Glutathione;    Oxidized phosphatidylethanolamine;    Ischemia-reperfusion;   

DOI  :  

来源: DOAJ

【 摘 要 】

Ferroptosis is a programmed iron-dependent cell death associated with peroxidation of lipids particularly, phospholipids. Several studies suggested a possible contribution of mitochondria to ferroptosis although the mechanisms underlying mitochondria-mediated ferroptotic pathways remain elusive. Reduced glutathione (GSH) is a central player in ferroptosis that is required for glutathione peroxidase 4 to eliminate oxidized phospholipids. Mitochondria do not produce GSH, and although the transport of GSH to mitochondria is not fully understood, two carrier proteins, the dicarboxylate carrier (DIC, SLC25A10) and the oxoglutarate carrier (OGC, SLC25A11) have been suggested to participate in GSH transport. Here, we elucidated the role of DIC and OGC as well as mitochondrial bioenergetics in ferroptosis in H9c2 cardioblasts. Results showed that mitochondria are highly sensitive to ferroptotic stimuli displaying fragmentation, and lipid peroxidation shortly after the onset of ferroptotic stimulus. Inhibition of electron transport chain complexes and oxidative phosphorylation worsened RSL3-induced ferroptosis. LC-MS/MS analysis revealed a dramatic increase in the levels of pro-ferroptotic oxygenated phosphatidylethanolamine species in mitochondria in response to RSL3 (ferroptosis inducer) and cardiac ischemia-reperfusion. Inhibition of DIC and OGC aggravated ferroptosis and increased mitochondrial ROS, membrane depolarization, and GSH depletion. Dihydrolipoic acid, an essential cofactor for several mitochondrial multienzyme complexes, attenuated ferroptosis and induced direct reduction of pro-ferroptotic peroxidized phospholipids to hydroxy-phospholipids in vitro. In conclusion, we suggest that ferroptotic stimuli diminishes mitochondrial bioenergetics and stimulates GSH depletion and glutathione peroxidase 4 inactivation leading to ferroptosis.

【 授权许可】

Unknown