Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short‐term paralysis: comparison of denervation and tetrodotoxin‐induced neural inactivation

FEBS Letters

Michel, Robin N.¹  Dunn, Shannon E.¹  Parry, David J.² 

[1] Neuromuscular Research Laboratory, Department of Chemistry and Biochemistry, Laurentian University, Ramsey Lake Road, Sudbury, Ontario P3E 2C6, Canada;Department of Physiology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada

关键词: Myosin;    Immunohistochemistry;    Activity;    Trophic mechanism;    Embryonic MHC;    α-Cardiac MHC;    ATPase;    adenosine triphosphatase;    Ig;    immunoglobulin;    EDL;    extensor digitorium longus;    BSA;    bovine serum albumin;    PBS;    phosphate-buffered saline;    DAB;    3;    3′-diaminobenzidine tetrahydrochloride;   

DOI  :  10.1016/0014-5793(96)00618-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The extent to which myosin profiles within adult fast and slow muscles are altered by short-term paralysis remains equivocal. We used an array of specific antibodies to identify adult and developmental MHC isoforms within EDL and soleus muscle fibers, and show a marked multiple expression of MHCs with a general shift towards slower and more energy efficient MHC profiles after 2 weeks of denervation or TTX nerve conduction block. Paralysis also induced marked expression of an embryonic MHC within most EDL cell types, and a subtle, paralysis-sensitive, expression of α-cardiac MHC within specific EDL and soleus extrafusal fibers. Comparison of treatment groups also permitted assessment of the relative influence of neural activity versus trophic factors on these isoforms, and confirmed activity as a major, but not sole, regulator of MHC expression.

【 授权许可】

Unknown