Unassembled subunits of the photosynthetic oxygen‐evolving complex present in the thylakoid lumen are long‐lived and assembly‐competent

FEBS Letters

Theg, Steven M.¹  Yasusi, Yamamoto²  Akiko, Hashimoto¹ 

[1] Section of Plant Biology, University of California, Davis, CA 95616, USA;Department of Biology, Okayama University, Okayama, Japan

关键词: Chloroplast;    Photosystem II;    Oxygen evolution;    Chl;    chlorophyll;    OEC;    oxygen-evolving complex;    OE33;    OE23 and OE17;    33;    23 and 17 kDa subunits of the OEC;    PS II;    photosystem II;   

DOI  :  10.1016/0014-5793(96)00686-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Physiologically healthy pea chloroplasts contain unassembled, soluble subunits of the oxygen-evolving complex in the thylakoid lumen. We report that the lifetimes of two of these subunits, both on and off the membrane, exceed 8 h in vitro. We also demonstrate that each of the subunits present in the thylakoid lumen is competent for assembly into the membrane-bound complex. These data are consistent with the postulate that the soluble lumen-resident subunits play a role in photosystem II homeostasis. We also demonstrate that the reconstitution of the 33 kDa subunit is inhibited by extremely low concentrations of Triton X-100, suggesting that hydrophobic interactions are involved in the binding of this subunit to the photosystem II reaction center.

【 授权许可】

Unknown