Isolation of a highly active PSII‐LHCII supercomplex from thylakoid membranes by a direct method

FEBS Letters

Eshaghi, Said²  Andersson, Bertil²  Barber, James¹ 

[1] Wolfson Laboratories, Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK;Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden

关键词: Oxygen evolution;    Photosystem II;    Thylakoid membrane;    CAB proteins;    chlorophyll a/b binding proteins;    Chl;    chlorophyll;    CP;    chlorophyll protein;    DM;    dodecyl-β-d-maltoside;    LHC;    light harvesting complex;    OEC;    oxygen evolving complex;    PMSF;    phenylmethylsulphonyl fluoride;    PSI;    photosystem I;    PSII;    photosystem II;   

DOI  :  10.1016/S0014-5793(99)00149-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have developed a simple and novel method to isolate a highly pure and active photosystem (PS) II complex, directly from thylakoid membranes. This complex is a discrete particle and contains all the proteins of the oxygen evolving complex and a set of chlorophyll a/b binding proteins. The intactness of both the donor side and the acceptor side has resulted in a very high oxygen evolution activity and therefore offers a superior experimental system to that of PSII enriched membrane fragments in which there is heterogeneity in activities and biochemical composition.

【 授权许可】

Unknown