In situ observation of streptavidin‐biotin binding on an immunoassay well surface using an atomic force microscope

FEBS Letters

Sefton, J.²  Williams, P.M.¹  Dawkes, A.C.²  Parker, M.C.¹  Allen, S.¹  Edwards, J.C.²  Tendler, S.J.B.¹  Davies, M.C.¹  Davies, J.²  Roberts, C.J.¹ 

[1] Laboratory of Biophysics and Surface Analysis, Department of Pharmaceutical Sciences, The University of Nottingham, University Park, Nottingham NG7 2RD, UK;Johnson and Johnson Clinical Diagnostics Ltd., Pollards Wood Laboratories, Nightingales Lane, Chalfont St Giles, Bucks. HP8 4SP, UK

关键词: Atomic force microscopy;    ELISA;    Force-distance curve;    Molecular interaction;    Streptavidin-biotin;   

DOI  :  10.1016/0014-5793(96)00651-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polystyrene microtitre wells are commonly used as supports for the enzyme-linked immunosorbent assay (ELISA) method of biomolecular detection, which is employed in the routine diagnosis of a variety of medical conditions. We have used an atomic force microscope (AFM) to directly monitor specific molecular interactions between individual streptavidin and biotin molecules on such wells. This was achieved by functionalising an AFM probe with biotin and monitoring the adhesive forces between the probe and a streptavidin coated immunoassay well. The results demonstrate that the AFM may be employed as an analytical tool to study the interactions between biomolecules involved in immunoassay systems.

【 授权许可】

Unknown