An electrophysiological study of calcium entry during normal human T‐lymphocyte activation

FEBS Letters

Ricci, Riccardo²  Buffelli, Mario³  Cangiano, Alberto³  Riviera, Anna P.¹ 

[1] Istituto di Immunologia e Malattie Infettive, Ospedale Borgo Roma, 37134 Verona, Italy;Istituto di Anatomia Patologica, Universita' Cattolica, Largo F. Vito 1, 00168 Roma, Italy;Dipartimento di Scienze Neurologiche e della Visione, Sezione di Fisiologia Umana, Universita' di Verona, Strada le Grazie 8, 37134 Verona, Italy

关键词: T-lymphocyte;    Calcium influx;    Patch-clamp technique;    Signal transduction;    Immune system;    Whole-cell recording;   

DOI  :  10.1016/0014-5793(96)00630-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Our aim was to observe whether normal human T-cells respond to mitogenic stimulation with large whole-cell inward currents (composed of identifiable single-channel contributions) when [Ca²⁺]_i is not markedly lowered but instead kept normal or moderately low, as has been reported in human leukaemic Jurkat T-cell line and T-cell clones [Kuno et al. (1986) Nature 323, 269–273; Kuno and Gardner (1987) Nature 326, 301–304; Gardner (1990) Annu. Rev. Immunol. 8, 231–252]. Whole-cell patch recordings showed no such currents in cells otherwise normally responding to depolarisation with the macroscopic I _K described in T-lymphocytes and thus deemed viable, in agreement with the notion that Ca²⁺ influx in normal T-cells enterily depends on depletion of internal stores [Putney (1986) Cell Calcium 7, 1–12; Putney (1990) Cell Calcium 11, 611–624].

【 授权许可】

Unknown