(+)‐Tubocurarine is a potent inhibitor of cation channels in the vacuolar membrane of Chenopodium rubrum L

FEBS Letters

Weiser, T.¹  Bentrup, F.-W.¹ 

[1] Botanisches Institut I der Justus-Liebig-Universität, Senckenbergstr. 17-21, D-6300 Giessen, Germany

关键词: Patch-clamp technique;    Plant vacuole;    Potassium channel;    Tonoplast;    (+)-Tubocurarine;    Chenopodium rubrum L;   

DOI  :  10.1016/0014-5793(90)80849-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of the acetylcholine antagonist and channel blocker (+)-tubocurarine on the calcium-dependent slow vacuolar (SV) cation channels in the tonoplast of suspension-cultured cells of Chenopodium rubrum L. was examined using the patch-clamp technique. In whole-vacuolar recordings the drug strongly suppressed the potassium conductance (EC₅₀: 6 μM) and altered the kinetics of channel inactivation. In excised membrane patches (+)-tuboeurarine evokes channel-‘flickering’ without affecting the single-channel conductance (approx. 80 pS).

【 授权许可】

Unknown